Microfluidics Lab

Faculty-In-Charge

Prof. Siddhartha Tripathi

Lab Overview

Microfluidics lab focuses on providing practical knowledge and experimental insights into understanding fluid flow at micro-scale, studying cell dynamics, & cell separation. The lab is designed to support cutting-edge research in the field of bio-microfluidics, enabling the development of Point-of care devices for biological and medical applications.

Key Research Areas

  • Bio-microfluidics
  • Cell separation
  • Cell dynamics
  • Point-of-Care microdevices

Significant Facilities & Special Features

  • PDMS-based microfluidic device fabrication using soft-lithography
  • Surface treatment and bonding using a plasma cleaner
  • Microscale flow visualization with microscopy
  • Cell counting

Major Equipment

Other facilities:

  • Hot air oven
  • PH measurement
  • Colorimeter
  • Vacuum pump
  • Laminar Air Flow
  • Medical Refrigerators

Research Funding & Grants

  • Microfluidics sperm cell sorting device: addressing male infertility factor (SERB-DST 2020-2022)
  • Isolation of white blood cells in a microfluidic device (ACRG BITS-Pilani 2019-21)
  • Isolation of white blood cells in a microfluidic device with applications to point-of-care diagnostics (BITS-BioCyTiH foundation (DST) 2022-24)
  • Microfluidic device for blood plasma separation and detection of Rheumatoid arthritis markers (CDRF BITS-Pilani 2024-25)

    • Achievements

    Publications:

  • Mane, S., Jacob, P., Hemadri, V., Dey, P., Bland, S., Tripathi, S., Characterization of the phagocytic ability of white blood cells separated using a single curvature spiral microfluidic device. Biomed. Eng. Lett. 2024
  • Mane, S., Behera, A., Hemadri, V., Bhand, S., Tripathi, S., Micropump integrated white blood cell separation platform for detection of chronic granulomatous disease. Microchim. Acta 191, 295, 2024
  • Puri, D.B., Kumar, S.S., Hemadri, V., Banerjee, A., Tripathi, S., Analysis of sperm cell motion in high viscosity Newtonian and non-Newtonian fluids using a microfluidic channel. Microfluid Nanofluid 28 (3),11, 2024
  • Behera, A., Mane, S., Hemadri, V., Bhand, S., Tripathi, S., White Blood Cell Separation for On-Chip Detection of Myeloperoxidase Activity Using a Microfluidic Platform. IEEE Sensors Letters, 7(12), 1-4, 2023
  • Mane, S., Hemadri, V. & Tripathi, S., Investigating WBC margination in different microfluidic geometries: Effect of RBC size and shape. Journal of micromechanics and Microengineering,33, 065002, 2023
  • Mane, S., Hemadri, V. & Tripathi, S., Separation of White Blood Cells in a Wavy Type Microfluidic Device Using Blood Diluted in a Hypertonic Saline Solution. BioChip J, 2022
  • Mane, N.S., Puri, D.B., Mane, S., Hemadri, V., Banerjee, A., Tripathi,S., Separation of motile human sperms in a T-shaped sealed microchannel. Biomed. Eng. Lett. 12, 331–342, 2022
  • Rajawat A., Tripathi, S., Disease diagnostics using hydrodynamic flow focusing in microfluidic devices: beyond flow cytometry, Biomed. Eng. Lett. 10, 241-257, 2020

Patents:

  • Mane, P. Jacob, V. hemadri, S. Bhand, S. Tripathi, Spiral microfluidic device for white blood cell separation from human blood, Indian Patent, Appl no. 20231104822

Recognition:

  • PROF. M.G. Deshpande Award BEST PAPER Award in Academia Effect of Newtonian and shear thinning medium on human sperm motion within a microchannel” Fluid Mechanics and Fluid Power, 48th National conference, 27th-29th Dec 2021
Campus
Goa
Division/Department
Goa-Mechanical Engineering